![Western Blot: MBP Antibody [NBP3-05553]](https://resources.rndsystems.com/images/products/MBP-Antibody-Western-Blot-NBP3-05553-img0003.jpg "Western Blot: MBP Antibody [NBP3-05553]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat
Format
BSA Free
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Product Specifications
Immunogen
Purified MBP isolated from bovine brain
Clonality
Polyclonal
Host
Goat
Scientific Data Images for MBP Antibody - BSA Free
Western Blot: MBP Antibody [NBP3-05553]
Western Blot: MBP Antibody [NBP3-05553] - Western blot analysis of different tissue lysates using MBP Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] mouse cerebellum, [4] cow midbrain and [5] pig midbrain. Multiple bands between 15-25 kDa mark correspond to the various alternate transcripts of the single MBP gene.![Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]](https://resources.rndsystems.com/images/products/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05553-img0002.jpg "Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]")
Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]
Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP3-05553] - Immunofluorescent analysis of mouse hippocampus section stained with MBP Antibody at a dilution of 1:5,000 in red, and costained with rabbit pAb to GFAP at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the GFAP antibody reveals the network of glial cells.![Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]](https://resources.rndsystems.com/images/products/MBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05553-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]")
Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP3-05553]
Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP3-05553] - Immunofluorescent analysis of mouse hippocampus section stained with MBP Antibody at a dilution of 1:5,000 in red, and costained with rabbit pAb to GFAP at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the GFAP antibody reveals the network of glial cells.Applications for MBP Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000-5000
Western Blot
1:5000-10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: MBP
Long Name
Myelin Basic Protein
Alternate Names
Hmbpr, mld, shi
Gene Symbol
MBP
Additional MBP Products
Product Documents for MBP Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MBP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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