MCAM/CD146 Antibody (P1H12) - BSA Free

Novus Biologicals | Catalog # NBP1-43346

Clone P1H12 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Rabbit, Rat (Negative)

Cited:

Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # P1H12

Format

BSA Free
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD146.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MCAM/CD146 Antibody (P1H12) - BSA Free

Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.
Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE.Total viable cells were used for analysis.
Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) - BSA Free [NBP1-43346]

Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Analysis using the FITC conjugate of NBP1-43346. Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-CD146 FITC (purple histogram). Right: Staining of normal human peripheral blood cells with Anti-Human CD3 APC and Anti-CD146 FITC.

Applications for MCAM/CD146 Antibody (P1H12) - BSA Free

Application
Recommended Usage

Flow Cytometry

< = 0.25 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCAM/CD146

The monoclonal antibody P1H12 recognizes human CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody (P1H12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MCAM/CD146 Antibody (P1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCAM/CD146 Antibody (P1H12) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MCAM/CD146 Antibody (P1H12) - BSA Free

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  • Q: Hello, I am looking at your antibody NBP1-43346APC, and I am curious what part of CD146 this antibody reacts to?

    A: The immunogen for clone P1H12 was Human Umbilical Vein Cells. Unfortunately the epitope was not mapped. We don't have any special instructions for permeabilization so we don't believe it is necessary. 

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