Mcl-1 Antibody (HL1544) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25560
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Canine, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1544 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Mcl-1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Mcl-1 Antibody (HL1544) - Azide and BSA Free (NBP3-25560) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Mcl-1 Antibody (HL1544) - Azide and BSA Free
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat tissue. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Immunohistochemistry-Paraffin: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - MCL1 antibody [HL1544] detects MCL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. MCL1 stained by MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] -
Western Blot: Mcl-1 Antibody (HL1544) - Azide and BSA Free [NBP3-25560] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody [HL1544] (NBP3-25560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Mcl-1 Antibody (HL1544) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Mcl-1
Long Name
Myeloid Cell Leukemia Sequence 1
Alternate Names
BCL2L3, Mcl1
Gene Symbol
MCL1
Additional Mcl-1 Products
Product Documents for Mcl-1 Antibody (HL1544) - Azide and BSA Free
Product Specific Notices for Mcl-1 Antibody (HL1544) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
