Mcl-1 Antibody (OTI10F6)

Novus Biologicals | Catalog # NBP2-45773

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10F6
View all Mcl-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mcl-1 Antibody (OTI10F6)

Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of prostate tissue.
Flow Cytometry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Flow Cytometry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Flow Cytometry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of HEK293T cells transfected with either pCMV6-ENTRY MCL1.
Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Western Blot: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of extracts (10ug).
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of breast tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Kidney tissue within the normal limits.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of liver tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of Carcinoma of pancreas tissue.
Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunohistochemistry: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of prostate tissue.
Immunofluorescence: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunofluorescence: Mcl-1 Antibody (OTI10F6) [NBP2-45773]

Immunofluorescence: Mcl-1 Antibody (OTI10F6) [NBP2-45773] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.

Applications for Mcl-1 Antibody (OTI10F6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:200 - 1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (OTI10F6)

Certificate of Analysis

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Product Specific Notices for Mcl-1 Antibody (OTI10F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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