MCM2 Antibody (2B3)

Novus Biologicals | Catalog # NBP2-37566

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B3
View all MCM2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MCM2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCM2 Antibody (2B3)

Western Blot: MCM2 Antibody (2B3)BSA Free [NBP2-37566]

Western Blot: MCM2 Antibody (2B3)BSA Free [NBP2-37566]

Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.
Immunohistochemistry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Immunohistochemistry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.
Flow Cytometry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Flow Cytometry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Flow Cytometry: MCM2 Antibody (2B3) [NBP2-37566] - Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).
Western Blot: MCM2 Antibody (2B3)BSA Free [NBP2-37566]

Western Blot: MCM2 Antibody (2B3)BSA Free [NBP2-37566]

Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)
Immunohistochemistry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Immunohistochemistry: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.
ELISA: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

ELISA: MCM2 Antibody (2B3) - BSA Free [NBP2-37566]

ELISA: MCM2 Antibody (2B3) [NBP2-37566] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for MCM2 Antibody (2B3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM2

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 Antibody (2B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MCM2 Antibody (2B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Citations for MCM2 Antibody (2B3)

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Protocols

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FAQs

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