MCM2 Antibody (6A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00004171-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6A8

Format

Azide and BSA Free
View all MCM2 Primary Antibodies »

Product Specifications

Immunogen

MCM2 (AAH07670, 805 a.a. ~ 904 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27976684).

Specificity

MCM2 - MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MCM2 Antibody (6A8) - Azide and BSA Free

Western Blot: MCM2 Antibody (6A8) [H00004171-M01]

Western Blot: MCM2 Antibody (6A8) [H00004171-M01]

Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01]

Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01]

Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MCM2 Antibody (6A8) [H00004171-M01]

Western Blot: MCM2 Antibody (6A8) [H00004171-M01]

Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.
Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01]

Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01]

Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.
ELISA: MCM2 Antibody (6A8) [H00004171-M01]

ELISA: MCM2 Antibody (6A8) [H00004171-M01]

ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.

Applications for MCM2 Antibody (6A8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCM2

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Entrez Gene IDs

4171 (Human)

Gene Symbol

MCM2

OMIM

116945 (Human)

UniProt

AAH07670 (Human)

Additional MCM2 Products

Product Documents for MCM2 Antibody (6A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MCM2 Antibody (6A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Citations for MCM2 Antibody (6A8) - Azide and BSA Free

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Protocols

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FAQs

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