MCM2 Antibody (BLR108H)

Novus Biologicals | Catalog # NBP3-07021

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rabbit

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR108H
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Product Specifications

Immunogen

surrounding Serine 53

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM2 Antibody (BLR108H)

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.
Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by ICC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.
Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.
Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji lysate. Antibody: Rabbit anti-Phospho-MCM2 recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021]

Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) by WB of BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021]

Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Applications for MCM2 Antibody (BLR108H)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate Buffered Saline (pH 8.2), 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

250 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: MCM2

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 Antibody (BLR108H)

Certificate of Analysis

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Product Specific Notices for MCM2 Antibody (BLR108H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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