MCM2 Antibody (S02-0D3)
Novus Biologicals | Catalog # NBP3-19887
Recombinant Monoclonal Antibody
![Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887]](https://resources.rndsystems.com/images/products/MCM2-Antibody-S02-0D3-Western-Blot-NBP3-19887-img0003.jpg "Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S02-0D3 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human MCM2 (Uniprot # P49736)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MCM2 Antibody (S02-0D3)
Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887]
Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.![Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887]](https://resources.rndsystems.com/images/products/MCM2-Antibody-S02-0D3-Immunohistochemistry-Paraffin-NBP3-19887-img0002.jpg "Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887]")
Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887]
Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunohistochemistry of MCM2 in paraffin-embedded Human tonsil using NBP3-19887 at dilution 1/50
Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] -
Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunocytochemistry of MCM2 (green) in hela using NBP3-19887 at dilution 1/5, and DAPI (blue)Applications for MCM2 Antibody (S02-0D3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/50
Immunohistochemistry
1/20
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MCM2
Long Name
Minichromosome Maintenance Protein 2
Alternate Names
BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin
Gene Symbol
MCM2
Additional MCM2 Products
Product Documents for MCM2 Antibody (S02-0D3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MCM2 Antibody (S02-0D3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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