MCM2 [p Ser108] Antibody - BSA Free

Novus Biologicals | Catalog # NB100-639

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM2 Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 108 according to the numbering given in entry NP_0045117.2 (GeneID 4171).

Modification

p Ser108

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM2 [p Ser108] Antibody - BSA Free

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody. Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).
Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639]

Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639]

Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639] - Human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for 2 hours. Antibody: Affinity purified rabbit anti-phospho MCM2 (Ser108) antibody (Cat. No. NB100-639) used at 0.2 mcg/ml. Subsequently, total MCM2 was detected to verify loading. Detection: Chemiluminescence with an exposure time of 5 seconds.
Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Whole cell lysate (15 or 50 ug) from 293T cells or mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibody used at 0.1 mcg/ml.

Applications for MCM2 [p Ser108] Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000- 1:5000

Immunohistochemistry-Paraffin

1:1000- 1:5000

Western Blot

1:5000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM2

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Entrez Gene IDs

4171 (Human)

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 [p Ser108] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MCM2 [p Ser108] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Citations for MCM2 [p Ser108] Antibody - BSA Free

Customer Reviews for MCM2 [p Ser108] Antibody - BSA Free

There are currently no reviews for this product. Be the first to review MCM2 [p Ser108] Antibody - BSA Free and earn rewards!

Have you used MCM2 [p Ser108] Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies