Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2068C

Format

Azide and BSA Free
View all MCM7 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human MCM-7 protein (amino acids 188-328) [UniProt P33993]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for MCM7 Antibody (2068C) - Azide and BSA Free

Western Blot: MCM7 Antibody (2068C)Azide and BSA Free [MAB92171]

Western Blot: MCM7 Antibody (2068C)Azide and BSA Free [MAB92171]

Western Blot: MCM7 Antibody (2068C) [MAB92171] - Analysis of HeLa, MCF-7, and MOLT-4, Daudi cell lysates with 0.5 ug/ml of hMCM7 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hMCM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Immunocytochemistry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Immunocytochemistry: MCM7 Antibody (2068C) [MAB92171] - MCM-7 was detected in immersion fixed HeLa cell using MCM-7 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Immunohistochemistry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Immunohistochemistry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Immunohistochemistry: MCM7 Antibody (2068C) [MAB92171] - MCM-7 was detected in immersion fixed paraffin-embedded sections of human mesothelioma using MCM-7 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.
Flow Cytometry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Flow Cytometry: MCM7 Antibody (2068C) - Azide and BSA Free [MAB92171]

Flow Cytometry: MCM7 Antibody (2068C) - Azide Free [MAB92171] - HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB92171, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for MCM7 Antibody (2068C) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM7

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Long Name

Minichromosome Maintenance Protein 7

Alternate Names

CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1

Gene Symbol

MCM7

Additional MCM7 Products

Product Documents for MCM7 Antibody (2068C) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MCM7 Antibody (2068C) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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FAQs

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