Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFIH1/MDA5 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5. Amino Acid Squence: FKNNSTKKQYKKWVEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDA5 Antibody - BSA Free

Western Blot: MDA5 AntibodyBSA Free [NBP1-76760]

Western Blot: MDA5 AntibodyBSA Free [NBP1-76760]

Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-76760]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-76760]

Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.
MDA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76760] -

Immunocytochemistry/ Immunofluorescence: MDA5 Antibody - BSA Free [NBP1-76760] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.
MDA5 Antibody - BSA Free

Immunohistochemistry: MDA5 Antibody - BSA Free [NBP1-76760] -

Immunohistochemistry: MDA5 Antibody - BSA Free [NBP1-76760] - Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 ug/ml.
MDA5 Antibody - BSA Free

Western Blot: MDA5 Antibody - BSA Free [NBP1-76760] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MDA5 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kappaB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

UniProt

NP_071451 (Human)

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MDA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

Citations for MDA5 Antibody - BSA Free

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Protocols

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FAQs

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