![Immunocytochemistry/ Immunofluorescence: MDM1 Antibody [NBP1-85839]](https://resources.rndsystems.com/images/products/MDM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85839-img0008.jpg "Immunocytochemistry/ Immunofluorescence: MDM1 Antibody [NBP1-85839]")
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKEN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MDM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: MDM1 Antibody [NBP1-85839]
Immunocytochemistry/Immunofluorescence: MDM1 Antibody [NBP1-85839] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centrosome.![MDM1 Antibody - BSA Free Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]](https://resources.rndsystems.com/images/products/nbp1-85839_rabbit-polyclonal-mdm1-antibody-23420251561920.jpg "Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]")
Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]
Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.![MDM1 Antibody - BSA Free Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]](https://resources.rndsystems.com/images/products/nbp1-85839_rabbit-polyclonal-mdm1-antibody-23420251573994.jpg "Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]")
Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]
Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.![MDM1 Antibody - BSA Free Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]](https://resources.rndsystems.com/images/products/nbp1-85839_rabbit-polyclonal-mdm1-antibody-2342025156195.jpg "Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]")
Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]
Staining of human rectum shows strong cytoplasmic positivity in glandular cells.![MDM1 Antibody - BSA Free Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]](https://resources.rndsystems.com/images/products/nbp1-85839_rabbit-polyclonal-mdm1-antibody-23420251573939.jpg "Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]")
Immunohistochemistry-Paraffin: MDM1 Antibody - BSA Free [NBP1-85839]
Staining of human liver shows negative to very weak cytoplasmic positivity in hepatocytes.![MDM1 Antibody - BSA Free Western Blot: MDM1 Antibody - BSA Free [NBP1-85839]](https://resources.rndsystems.com/images/products/nbp1-85839_rabbit-polyclonal-mdm1-antibody-2442025942170.jpg "Western Blot: MDM1 Antibody - BSA Free [NBP1-85839]")
Western Blot: MDM1 Antibody - BSA Free [NBP1-85839]
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Applications for MDM1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 4 using NBP1-85839 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MDM1
Alternate Names
FLJ95264, Mdm1 nuclear protein homolog (mouse), Mdm4, transformed 3T3 cell double minute 1, p53 binding protein, Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse), nuclear protein double minute 1, nuclear protein MDM1
Gene Symbol
MDM1
Additional MDM1 Products
Product Documents for MDM1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MDM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for MDM1 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
Have you used MDM1 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
-(01-ml)_NBP1-85839_8696.jpg)
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: 293T lysatesSpecies: HumanVerified Customer | Posted 07/02/2014WB for MDM1 in 293T cells
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MDM1 Antibody - BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: I used an Ab NBP-1 85839 from your company in NSLC cells for western and am wondering what the expected size of this protein should be.
A: The MDM1 protein has an expected molecular weight of 80kDa. There are four isoforms to this protein and our product NBP1-85839 is capable of detecting isoforms 1 and 4 (80 and 76kDa respectively). uniprot site In western blot, our lab has detected bands from 70-78kDa. Since there is a phophoserine residue at amino acid 83, it is possible to detect bands up to 80-85kDa.
Loading...