MDR1/ABCB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90291

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MDR1/ABCB1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDR1/ABCB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291]

Immunocytochemistry/ Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291]

Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.
MDR1/ABCB1 Antibody - BSA Free Immunohistochemistry: MDR1/ABCB1 Antibody - BSA Free [NBP1-90291]

Immunohistochemistry: MDR1/ABCB1 Antibody - BSA Free [NBP1-90291]

Staining of human adrenal gland shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.

Applications for MDR1/ABCB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDR1/ABCB1

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Gene Symbol

ABCB1

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MDR1/ABCB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MDR1/ABCB1 Antibody - BSA Free

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Protocols

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