MeCP2 Antibody (4B6) - Azide and BSA Free

Novus Biologicals | Catalog # H00004204-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B6

Format

Azide and BSA Free
View all MeCP2 Primary Antibodies »

Product Specifications

Immunogen

MECP2 (AAH11612, 81 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Nucleus Marker

Specificity

MECP2 - methyl CpG binding protein 2 (Rett syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MeCP2 Antibody (4B6) - Azide and BSA Free

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01]

Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01]

Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6 Analysis of MECP2 expression in MCF-7.
Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in NIH/3T3.
Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01]

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in rat muscle.
Sandwich ELISA: MeCP2 Antibody (4B6) [H00004204-M01] - Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.

Applications for MeCP2 Antibody (4B6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MeCP2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Entrez Gene IDs

4204 (Human)

Gene Symbol

MECP2

OMIM

105830 (Human)

UniProt

AAH11612 (Human)

Additional MeCP2 Products

Product Documents for MeCP2 Antibody (4B6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MeCP2 Antibody (4B6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MeCP2 Antibody (4B6) - Azide and BSA Free

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Protocols

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FAQs

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