MeCP2 Antibody (5H12) - BSA Free

Novus Biologicals | Catalog # NBP2-60787

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5H12

Format

BSA Free
View all MeCP2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant MeCP2. Accession # P51608

Specificity

Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MeCP2 Antibody (5H12) - BSA Free

Western Blot: MeCP2 Antibody (5H12) [NBP2-60787]

Western Blot: MeCP2 Antibody (5H12) [NBP2-60787]

Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.
Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (1:500, red), colabeled with anti-GFAP (1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Rat hippocampal section showing specific nuclear MeCP2immunolabeling in red and FOX3 in green.
Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787]

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (1:1000, red) in nuclei of neurons and specific labeling of GFAP (1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.

Applications for MeCP2 Antibody (5H12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MeCP2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Gene Symbol

MECP2

Additional MeCP2 Products

Product Documents for MeCP2 Antibody (5H12) - BSA Free

Certificate of Analysis

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Product Specific Notices for MeCP2 Antibody (5H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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