MED25 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55868

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED25 Antibody - BSA Free

Western Blot: MED25 Antibody [NBP2-55868]

Western Blot: MED25 Antibody [NBP2-55868]

Western Blot: MED25 Antibody [NBP2-55868] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: MED25 Antibody [NBP2-55868]

Immunocytochemistry/ Immunofluorescence: MED25 Antibody [NBP2-55868]

Immunocytochemistry/Immunofluorescence: MED25 Antibody [NBP2-55868] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.
MED25 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MED25 Antibody - BSA Free [NBP2-55868]

Chromatin Immunoprecipitation-exo-Seq: MED25 Antibody - BSA Free [NBP2-55868]

ChIP-Exo-Seq composite graph for Anti-MED25 (NBP2-55868) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
MED25 Antibody - BSA Free

Western Blot: MED25 Antibody - BSA Free [NBP2-55868] -

Replication of RSV B1 is significantly enhanced in MED25 knockout A549 cells. (A) The lack of expression of MED25 protein in knockout cells was analyzed by Western blotting using an anti-MED25 antibody. GAPDH staining was used as loading control. (B) MED25 knockout (ko #1, ko #2, and ko #3), parental (par), and wild-type (wt) A549 cells were infected with RSV B1 (MOI, 0.005). For 5 days postinfection, supernatant was collected daily for virus titration in wild-type A549 cells by using plaque assays. PFU/mL data from two independent experiments, each performed in triplicate, were analyzed using a generalized linear mixed model (see “Statistical analysis of replication kinetics"). The scale of the y axis is based upon a natural logarithm. ***, P < 0.005; *, P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36102648), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MED25 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED25

Alternate Names

ACID1, Activator Interaction Domain-Containing Protein 1, Activator-Recruited Cofactor 92 KDa Component, ARC/Mediator Transcriptional Coactivator Subunit, ARC92, BVSYS, CMT2B2, Mediator Complex Subunit 25, Mediator Of RNA Polymerase II Transcription Subunit 25, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae), P78, TCBAP0758

Gene Symbol

MED25

Additional MED25 Products

Product Documents for MED25 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MED25 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MED25 Antibody - BSA Free

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Protocols

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FAQs

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