MED4 Antibody (8T5C9)

Novus Biologicals | Catalog # NBP3-15287

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8T5C9 expressed in HEK293
View all MED4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human MED4 (Q9NPJ6). KYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED4 Antibody (8T5C9)

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287]

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287]

Western Blot: MED4 Antibody (8T5C9) [NBP3-15287] - Western blot analysis of extracts of various cell lines, using MED4 antibody (NBP3-15287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry of paraffin-embedded rat liver using MED4 Rabbit mAb (NBP3-15287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MED4 Rabbit mAb (NBP3-15287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287]

Immunohistochemistry-Paraffin: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry of paraffin-embedded mouse lung using MED4 Rabbit mAb (NBP3-15287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded rat colon tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded human breast tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded human colon tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded mouse colon tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded human esophagus tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MED4 Antibody (8T5C9)

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] -

Immunohistochemistry: MED4 Antibody (8T5C9) [NBP3-15287] - Immunohistochemistry analysis of MED4 in paraffin-embedded human breast cancer tissue using MED4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for MED4 Antibody (8T5C9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MED4

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Gene Symbol

MED4

Additional MED4 Products

Product Documents for MED4 Antibody (8T5C9)

Certificate of Analysis

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Product Specific Notices for MED4 Antibody (8T5C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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