MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-20686
![MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-2711202419553094.jpg "Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
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Product Specifications
Immunogen
MEK1/2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/2.
Modification
p Ser221, p Ser217
Specificity
This antibody detects endogenous level of MEK1/2 only when phosphorylated at serine 217/221.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -
Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).![MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free](https://resources.rndsystems.com/images/products/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-271120242074623.jpg "Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -")
Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] -
Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/2 (p Ser221, p Ser217)Applications for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:100
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Epitope affinity purified
Formulation
PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: MEK1/MEK2
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbol
MAP2K1
Additional MEK1/MEK2 Products
Product Documents for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
