MEKK4 Antibody (23GB4045)

Novus Biologicals | Catalog # NBP3-50312

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4045
View all MEKK4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MEKK4 (Uniprot: Q9Y6R4)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEKK4 Antibody (23GB4045)

MEKK4 Antibody (23GB4045)

Immunocytochemistry/ Immunofluorescence: MEKK4 Antibody (23GB4045)[NBP3-50312] -

Immunocytochemical staining of C2C12 cells with MEKK4 antibody (1:1,000). Nuclei were stained blue with DAPI; MEKK4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MEKK4 Antibody (23GB4045)

Flow Cytometry: MEKK4 Antibody (23GB4045)[NBP3-50312] -

Flow cytometric analysis of MEKK4expression in C2C12 cells using MEKK4 antibody (1:2,000). Green, isotype control; red, MAP3K4.
MEKK4 Antibody (23GB4045)

Western Blot: MEKK4 Antibody (23GB4045)[NBP3-50312] -

Western blotting analysis using MEKK4 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for MEKK4 Antibody (23GB4045)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEKK4

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Alternate Names

EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4

Gene Symbol

MAP3K4

Additional MEKK4 Products

Product Documents for MEKK4 Antibody (23GB4045)

Certificate of Analysis

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Product Specific Notices for MEKK4 Antibody (23GB4045)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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