Melanocortin-5 R/MC5R Antibody

Novus Biologicals | Catalog # NBP1-36971

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Melanocortin-5 R/MC5R Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence PNVKNKSSPCED corresponding to N-Terminus (near) according to NP_005904.1.

Epitope

N Terminus (near)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Melanocortin-5 R/MC5R Antibody

Western Blot: Melanocortin-5 R/MC5R Antibody [NBP1-36971]

Western Blot: Melanocortin-5 R/MC5R Antibody [NBP1-36971]

Western Blot: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - Analysis of MC5 Receptor in Mouse Muscle lysate (35ug protein in RIPA buffer) using NBP1-36971 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Melanocortin-5 R/MC5R Antibody [NBP1-36971]

Immunohistochemistry-Paraffin: Melanocortin-5 R/MC5R Antibody [NBP1-36971]

Immunohistochemistry-Paraffin: Melanocortin-5 R/MC5R Antibody [NBP1-36971] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Melanocortin-5 R/MC5R Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 36.6 kDa band according to NP_005904.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Melanocortin-5 R/MC5R

The melanocortin family comprises the alpha-, beta- and gamma-melanocyte stimulating hormones (MSH) and adrenocorticotrophin. The receptors for these hormones are seven-transmembrane, G protein-coupled proteins that activate adenylyl cyclase. Five melanocortin receptors have been cloned and shown to exhibit different affinities and patterns of expression. MC1-R (MSH-R) is expressed in melanocytes and corticoadrenal tissue. MC2-R is the ACTH receptor and is expressed primarily in the adrenal cortex. MC3-R has been found in specific regions of the brain and is also expressed in placenta and gut. MC4-R is expressed primarily in brain, while MC5-R is expressed at low levels in most tissues.

Long Name

Melanocortin 5 Receptor

Alternate Names

MC2, MC5, MC5R, Melanocortin-5R, Melanocortin5R

Entrez Gene IDs

4161 (Human); 17203 (Mouse)

Gene Symbol

MC5R

UniProt

NP_005904.1 (Human)

Additional Melanocortin-5 R/MC5R Products

Product Documents for Melanocortin-5 R/MC5R Antibody

Certificate of Analysis

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Product Specific Notices for Melanocortin-5 R/MC5R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Melanocortin-5 R/MC5R Antibody

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Protocols

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