Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02)

Western Blot: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589]

Western Blot: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Western blot analysis of Melanoma HMB45 + MART1 + Tyrosinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperat

Immunohistochemistry-Paraffin: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589]

Immunohistochemistry-Paraffin: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Immunohistochemical analysis of paraffin-embedded human melanoma tissue using anti-Melanoma HMB45 + MART1 + Tyrosinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589]

Flow Cytometry: Melanoma HMB45 + MART1 + Tyrosinase Antibody (SC56-02) [NBP2-67589] - Analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody