Melanotransferrin/CD228/MFI2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85777

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Melanotransferrin/CD228 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

Reactivity Notes

Mouse (87%), Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Melanotransferrin/CD228/MFI2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Analysis in human salivary gland and skeletal muscle tissues. Corresponding MELTF RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human skin shows weak to moderate cytoplasmic positivity in keratinocytes.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human salivary gland shows moderate to strong positivity in luminal membrane in glandular cells.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human kidney shows moderate to strong membranous positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777]

Immunohistochemistry-Paraffin: Melanotransferrin/CD228/MFI2 Antibody [NBP1-85777] - Staining of human skeletal muscle shows no positivity in myocytes.
Melanotransferrin/CD228/MFI2 Antibody - BSA Free Western Blot: Melanotransferrin/CD228/MFI2 Antibody - BSA Free [NBP1-85777]

Western Blot: Melanotransferrin/CD228/MFI2 Antibody - BSA Free [NBP1-85777]

Analysis in human cell lines SK-MEL-30 and HeLa using Anti-MELTF antibody. Corresponding MELTF RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Applications for Melanotransferrin/CD228/MFI2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Melanotransferrin/CD228

MFI2 is encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

Alternate Names

CD228, MAP97, MFI2, MTF, p97

Gene Symbol

MELTF

Additional Melanotransferrin/CD228 Products

Product Documents for Melanotransferrin/CD228/MFI2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Melanotransferrin/CD228/MFI2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Melanotransferrin/CD228/MFI2 Antibody - BSA Free

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Protocols

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