Metadherin Antibody (2F11C3) - BSA Free

Novus Biologicals | Catalog # NBP1-51585

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F11C3

Format

BSA Free
View all Metadherin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Metadherin expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Metadherin Antibody (2F11C3) - BSA Free

Western Blot: Metadherin Antibody (2F11C3)BSA Free [NBP1-51585]

Western Blot: Metadherin Antibody (2F11C3)BSA Free [NBP1-51585]

Western Blot: LYRIC Antibody (2F11C3) [NBP1-51585] - Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Immunohistochemistry-Paraffin: Metadherin Antibody (2F11C3) - BSA Free [NBP1-51585]

Immunohistochemistry-Paraffin: Metadherin Antibody (2F11C3) - BSA Free [NBP1-51585]

Immunohistochemistry-Paraffin: LYRIC Antibody (2F11C3) [NBP1-51585] - Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Flow Cytometry: Metadherin Antibody (2F11C3) - BSA Free [NBP1-51585]

Flow Cytometry: Metadherin Antibody (2F11C3) - BSA Free [NBP1-51585]

Flow Cytometry: LYRIC Antibody (2F11C3) [NBP1-51585] - Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).

Applications for Metadherin Antibody (2F11C3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascitic fluid

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Metadherin

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumor tissues.

Alternate Names

3D3, AEG1, LYRIC, MTDH

Gene Symbol

MTDH

Additional Metadherin Products

Product Documents for Metadherin Antibody (2F11C3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Metadherin Antibody (2F11C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
NFkB Pathway

Citations for Metadherin Antibody (2F11C3) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Metadherin Antibody (2F11C3) - BSA Free

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  • Q: Could you give me some details about the NBP1-51585? Especially the epitope, I really want to know it exactly.

    A: The exact immunogen sequence is proprietary, but the immunogen range is between 378-579. The antibody has not been epitope mapped to our knowledge.

  • Q: I was just wondering whether the HeLa cells used in the flow cytometry were permeabilised or not? As I am interested in looking whether LYRIC is surface expressed on my cell type.

    A: The HeLa cells were permeabilised by using 0.1% Triton 100.

  • Q: Could you give me some details about the NBP1-51585? Especially the epitope, I really want to know it exactly.

    A: The exact immunogen sequence is proprietary, but the immunogen range is between 378-579. The antibody has not been epitope mapped to our knowledge.

  • Q: I was just wondering whether the HeLa cells used in the flow cytometry were permeabilised or not? As I am interested in looking whether LYRIC is surface expressed on my cell type.

    A: The HeLa cells were permeabilised by using 0.1% Triton 100.

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