Metadherin Antibody (CL0401) - BSA Free

Novus Biologicals | Catalog # NBP2-30458

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0401

Format

BSA Free
View all Metadherin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q86UE4, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Metadherin Antibody (CL0401) - BSA Free

Western Blot: Metadherin Antibody (CL0401) [NBP2-30458]

Western Blot: Metadherin Antibody (CL0401) [NBP2-30458]

Western Blot: LYRIC Antibody (CL0401) [NBP2-30458] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Metadherin Antibody (CL0401) [NBP2-30458]

Immunocytochemistry/ Immunofluorescence: Metadherin Antibody (CL0401) [NBP2-30458]

Immunocytochemistry/Immunofluorescence: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of U-251 cells using the Anti-MTDH monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human placenta shows absence of staining in the trophoblast layer (negative control).
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human testis shows strong cytoplasmic immunoreactivity in the seminiferous tubules.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human colon shows moderate cytoplasmic positivity in the glandular cells.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human colorectal cancer shows moderate cytoplasmic immunoreactivity in cancer cells.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human breast cancer (ductal carcinoma) shows strong cytoplasmic positivity in cancer cells.
Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: Metadherin Antibody (CL0401) [NBP2-30458]

Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate cytoplasmic immunoreactivity in cancer cells.

Applications for Metadherin Antibody (CL0401) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Metadherin

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues.

Alternate Names

3D3, AEG1, LYRIC, MTDH

Gene Symbol

MTDH

UniProt

Q86UE4

Additional Metadherin Products

Product Documents for Metadherin Antibody (CL0401) - BSA Free

Certificate of Analysis

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Product Specific Notices for Metadherin Antibody (CL0401) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
NFkB Pathway

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Protocols

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FAQs

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