metallothionein Antibody (UC1MT) - BSA Free

A:

I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

A:

This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.

A: Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).

A:

I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

A:

This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.

A: Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).

A:

I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

A:

This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.

A: Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).