Methionine Aminopeptidase 2/METAP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05513

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Methionine Aminopeptidase 2/METAP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionine Aminopeptidase 2/METAP2 Antibody - BSA Free

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-METAP2 antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunocytochemistry/ Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Cervix shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513]

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Tonsil shows strong cytoplasmic positivity in germinal center cells.

Applications for Methionine Aminopeptidase 2/METAP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Aminopeptidase 2/METAP2

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Alternate Names

Amp2, METAP2, MNPEP, p67eIF2

Gene Symbol

METAP2

Additional Methionine Aminopeptidase 2/METAP2 Products

Product Documents for Methionine Aminopeptidase 2/METAP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Methionine Aminopeptidase 2/METAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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