METTL14 Antibody - BSA Free

Immunohistochemistry-Paraffin: METTL14 Antibody [NBP1-81392]

Immunohistochemistry-Paraffin: METTL14 Antibody [NBP1-81392] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Simple Western: METTL14 Antibody [NBP1-81392]

Simple Western: METTL14 Antibody [NBP1-81392] - Simple Western lane view shows a specific band for METTL14 in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: METTL14 Antibody - BSA Free [NBP1-81392] -

Knockdown of METTL14 or inhibition of m6A methylation modification can partially rescue the decline in cell proliferation induced by atRA. qRT-PCR (A) and Western blot (B) detection of the siRNA knockdown efficiency of METTL14. (C) Dot blotting detected the m6A methylation modification level of MEPM cells after treatment with siMETTL14 and atRA. (D) Flow cytometry to detect MEPM cell cycle changes after siMETTL14 and atRA treatments. (E) Western blot detected changes in MEPM cell cycle and proliferation-related proteins after siMETTL14 and atRA treatments. (F) Dot blotting detected m6A methylation modification level after SAH treatment of MEPM. (G) Western blot detection of METTL14 protein expression after SAH treatment of MEPM. (H) CCK8 experiment to detect cell proliferation after SAH and atRA treatments of MEPM. n.s is considered not statistically significant, * means p < 0.05, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: METTL14 Antibody - BSA Free [NBP1-81392] -

Increased m6A level and expression of METTL14 in embryonic palatal mesenchyme were associated with cleft palate. (A) Dot blot was used to detect the m6A modification level of RNA in the palatal mesenchyme of embryonic mice in the control group, RA10D group, and RA12D group. (B) qRT-PCR detection of the expression of m6A methylation modification enzymes in the palatal mesenchyme of embryonic mice in the control group and RA10D group. (C) qRT-PCR detection of METTL14 and WTAP mRNA expression and changes in the palatal mesenchyme of the control group and RA10D group on days E13.5–E15.5. (D) Western blot detection of METTL14 expression in the palatal mesenchyme of embryonic mice in the control group and RA10D group. (E,F) Immunofluorescence detection and semi-quantitative analysis of METTL14 expression in the palatal mesenchyme of embryonic mice in the control group and RA10D group from E13.5 to E15.5 days (green: METTL14, blue: DAPI) (10× magnification). n.s is considered not statistically significant, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: METTL14 Antibody - BSA Free [NBP1-81392] -

Knockdown of METTL14 or inhibition of m6A methylation modification can partially rescue the decline in cell proliferation induced by atRA. qRT-PCR (A) and Western blot (B) detection of the siRNA knockdown efficiency of METTL14. (C) Dot blotting detected the m6A methylation modification level of MEPM cells after treatment with siMETTL14 and atRA. (D) Flow cytometry to detect MEPM cell cycle changes after siMETTL14 and atRA treatments. (E) Western blot detected changes in MEPM cell cycle and proliferation-related proteins after siMETTL14 and atRA treatments. (F) Dot blotting detected m6A methylation modification level after SAH treatment of MEPM. (G) Western blot detection of METTL14 protein expression after SAH treatment of MEPM. (H) CCK8 experiment to detect cell proliferation after SAH and atRA treatments of MEPM. n.s is considered not statistically significant, * means p < 0.05, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: METTL14 Antibody - BSA Free [NBP1-81392] -

Increased m6A level and expression of METTL14 in embryonic palatal mesenchyme were associated with cleft palate. (A) Dot blot was used to detect the m6A modification level of RNA in the palatal mesenchyme of embryonic mice in the control group, RA10D group, and RA12D group. (B) qRT-PCR detection of the expression of m6A methylation modification enzymes in the palatal mesenchyme of embryonic mice in the control group and RA10D group. (C) qRT-PCR detection of METTL14 and WTAP mRNA expression and changes in the palatal mesenchyme of the control group and RA10D group on days E13.5–E15.5. (D) Western blot detection of METTL14 expression in the palatal mesenchyme of embryonic mice in the control group and RA10D group. (E,F) Immunofluorescence detection and semi-quantitative analysis of METTL14 expression in the palatal mesenchyme of embryonic mice in the control group and RA10D group from E13.5 to E15.5 days (green: METTL14, blue: DAPI) (10× magnification). n.s is considered not statistically significant, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: METTL14 Antibody - BSA Free [NBP1-81392] -

Inhibition of mesenchymal cell proliferation in cleft palate mice may be related to the abnormal expression of METTL14. (A) Western blot detection of the proliferation and cell cycle-related differential genes of palatal mesenchyme cells in the control group and RA10D cleft palate group on days E13.5, E14.5, and E15.5. ki67 immunofluorescence detection of cell proliferation levels in the palatal mesenchyme of embryonic mice in the control and RA10D/R zA12D cleft palate groups on days E13.5 (B), E14.5 (C), and E15.5 (D) (green: ki67, blue: DAPI) (10× magnification). (E) Immunohistochemical detection of METTL14 and PCNA expression in the palatal mesenchyme of embryonic mice in the control group and RA10D cleft palate group. n.s is considered not statistically significant, * means p < 0.05, ** means p < 0.01, *** means p < 0.001, **** means p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.