mGluR1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-93555

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all mGluR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide comprising internal residues of the human metabotropic GluR1 alpha protein. Reacts with rat and mouse GluR1.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Specificity

mGluR1, Synthetic peptide comprising internal residues of the human GluR1 alpha protein. Reacts with rat and mouse mGluR1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit mGluR1 Antibody - BSA Free (NB100-93555) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-mGluR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for mGluR1 Antibody - BSA Free

Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

mGluR1-Antibody-Immunohistochemistry-NB100-93555-img0006.jpg
Western Blot: mGluR1 AntibodyBSA Free [NB100-93555]

Western Blot: mGluR1 AntibodyBSA Free [NB100-93555]

Western Blot: mGluR1 Antibody [NB100-93555] - Detection of metabotropic Glutamate Receptor 1a protein in 20ug of human brain lysate (lane 2) with the use of NB100-93555 as a primary antibody at 1:1000 dilution and an AP conjugated secondary antibody at 1:5000 dilution. Lane 1 represents the MW marker.
Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

Immunohistochemistry: mGluR1 Antibody [NB100-93555] - Recommended dilution for IHC is 1:100-500. However, the investigator should determine the optimal dilution for a specific application.
Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

Immunohistochemistry: mGluR1 Antibody - BSA Free [NB100-93555]

mGluR1-Antibody-Immunohistochemistry-NB100-93555-img0004.jpg
mGluR1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB100-93555] -

Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB100-93555] - Immunohistochemical staining of mGluR1 alpha & MAP2. Neurons of the dLGN were stained using specific antibodies against microtubule associated protein 2 (MAP2) & against mGluR1 alpha conjugated to Cy2 & Cy3, respectively. While MAP2 was clearly present in the soma, mGluR1 alpha appears to be absent there. Scale bars indicate 10 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fncel.2010.00132/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for mGluR1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Western Blot

1:500-1:1000
Application Notes
This antibody is useful for Western blot 1:500-1:1000, and immunohistochemistry 1:100-1:500. Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Frozen were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mGluR1

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. The Glutamate Receptor, Metabotropic, 1, (mGluR1 alpha) activates phospholipase C and participates in the central action of glutamate in the central nervous system, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for mGluR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for mGluR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for mGluR1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for mGluR1 Antibody - BSA Free

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  • Q: Is your antibody NB100-93555 directed at Q13255 or P42261?

    A: NB100-93555 is directed against metabotropic Glutamate Receptor 1a, also known as GRM1, GPRC1A, and MGLUR1. This corresponds to UniProt Q13255.

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