mGluR5/1 Antibody - Azide Free

Novus Biologicals | Catalog # NB300-126

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all mGluR5/1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P31424

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15608650).

Localization

Cell Membrane

Specificity

Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa dimers of mGluR5 and mGluR1. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mGluR5/1 Antibody - Azide Free

Western Blot: mGluR5/1 Antibody [NB300-126]

Western Blot: mGluR5/1 Antibody [NB300-126]

Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) labeling the ~125k monomer and the ~250k dimers for both mGluRs.

Applications for mGluR5/1 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30868621).

Reviewed Applications

Read 1 review rated 5 using NB300-126 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mGluR5/1

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Alternate Names

glutamate receptor, metabotropic 5, GPRC1EMGLUR5metabotropic glutamate receptor 5, mGlu5, mGluR5

Entrez Gene IDs

24418 (Rat)

Gene Symbol

GRM5

UniProt

P31424 (Rat)

Additional mGluR5/1 Products

Product Documents for mGluR5/1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for mGluR5/1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for mGluR5/1 Antibody - Azide Free

Customer Reviews for mGluR5/1 Antibody - Azide Free (1)

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Customer Images

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  • metabotropic Glutamate Receptor 5/1 Antibody
    Name: Daniel Popoola
    Application: Western Blot
    Sample Tested: brain
    Species: Rat
    Verified Customer | Posted 03/21/2017
    Western Blot analysis of adult female rat brain whole-cell homogenates. Row A represents mGluR5/1 monomers while row B represent dimers.
    mGluR5/1 Antibody - Azide Free NB300-126

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