mGluR5/1a Antibody (S75-33) - BSA Free
Novus Biologicals | Catalog # NBP2-59334
![Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334]](https://resources.rndsystems.com/images/products/mGluR5-1a-Antibody-S75-33-Western-Blot-NBP2-59334-img0005.jpg "Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S75-33
Format
BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
Localization
Cell Membrane
Specificity
Detects 130kDa (both mGluR1 and mGluR5).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for mGluR5/1a Antibody (S75-33) - BSA Free
Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334]
Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5/1a Glutamate Receptor protein using Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody, Clone S75-33 (NBP2-59334). Primary Antibody: Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody (NBP2-59334) at 1:1000.![Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]](https://resources.rndsystems.com/images/products/mGluR5-1a-Antibody-S75-33-Immunocytochemistry-Immunofluorescence-NBP2-59334-img0006.jpg "Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]")
Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]
Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33 (NBP2-59334). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-59334) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.![Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]](https://resources.rndsystems.com/images/products/mGluR5-1a-Antibody-S75-33-Immunocytochemistry-Immunofluorescence-NBP2-59334-img0002.jpg "Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]")
Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]
Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.Applications for mGluR5/1a Antibody (S75-33) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: mGluR5/1a
Long Name
Metabotropic Glutamate Receptor 5 and 1
Additional mGluR5/1a Products
Product Documents for mGluR5/1a Antibody (S75-33) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for mGluR5/1a Antibody (S75-33) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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