MHC Class II Antibody (ER-TR2) - BSA Free

Immunohistochemistry: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959] -

Development of CALT under SPF housing conditions.A) Immunhistological analysis of conjunctiva-associated lymphoid tissue (CALT). Mice aged 10 days until 24 weeks, kept under SPF housing conditions, were investigated using a panel of antibodies as described in table 1. Spatial distribution of lymphocytes, dendritic cells and follicular dendritic cells was analyzed. B) Schematic CALT development: 10 days after birth only CD4+ T-cells were sparsely present, followed by an influx of B-cells and formation of first follicles at 4 weeks of age. At 8 to 12 weeks CD8+ T-cells and CD4+CD25+ Tregs appeared, altogether forming a complex lymphoid follicle. After 16 weeks of age spatial organization diminished. C) CALT expression rate: CALT was not present at 10 days of age. 25% of the eyes at 4 weeks of age contained CALT, with a further increase to 43% CALT at 10 and 12 weeks of age. This was followed by a decrease to 31% at 16 weeks and an increased to 50% at 24 weeks of age. Numbers of follicles increased until 25% of the eyes contained 2 follicles at 12 weeks of age. At 24 weeks of age 25% of the eyes contained 3 follicles (n = number of eyes). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24376530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959] -

CALT under standard housing conditions.Immunhistological staining of CALT at 10–24 weeks that were kept under standard housing conditions were investigated using a panel of antibodies as described in table 1. Spatial distribution of lymphocytes, dendritic cells and follicular dendritic cells was analyzed. A) Comparison of CALT expression in mice kept under standard housing conditions with concordant time points of animals kept under SPF housing conditions (expression rates from figure 1). Statistically CALT expression was similar in both housing conditions. (SPF = specific pathogen free; SH = Standard housing conditions; n = number of eyes examined. Differences were statistically not significant, p>0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24376530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.