Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3G7
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-296 of human MIER2(NP_060020) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
59.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MIER2 Antibody (OTI3G7)
![Western Blot: MIER2 Antibody (OTI3G7) [NBP2-01748]](https://resources.rndsystems.com/images/products/MIER2-Antibody-3G7-Western-Blot-NBP2-01748-img0001.jpg "Western Blot: MIER2 Antibody (OTI3G7) [NBP2-01748]")
Western Blot: MIER2 Antibody (OTI3G7) [NBP2-01748]
Western Blot: MIER2 Antibody (3G7) [NBP2-01748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIER2.
Immunohistochemistry-Paraffin: MIER2 Antibody (3G7) [NBP2-01748] Staining of paraffin-embedded Human tonsil using anti-MIER2 mouse monoclonal antibody.
![Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]](https://resources.rndsystems.com/images/products/MIER2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-01748-img0002.jpg "Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]")
Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]
Immunohistochemistry-Paraffin: MIER2 Antibody (3G7) [NBP2-01748] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MIER2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]](https://resources.rndsystems.com/images/products/MIER2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-01748-img0003.jpg "Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]")
Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]
Immunohistochemistry-Paraffin: MIER2 Antibody (3G7) [NBP2-01748] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MIER2 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]](https://resources.rndsystems.com/images/products/MIER2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-01748-img0004.jpg "Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]")
Immunohistochemistry-Paraffin: MIER2 Antibody (OTI3G7) [NBP2-01748]
Immunohistochemistry-Paraffin: MIER2 Antibody (3G7) [NBP2-01748] - Staining of paraffin-embedded Human endometrium tissue using anti-MIER2 mouse monoclonal antibody.Applications for MIER2 Antibody (OTI3G7)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MIER2
Alternate Names
KIAA1193mesoderm induction early response protein 2, mesoderm induction early response 1, family member 2, Mi-er2
Entrez Gene IDs
54531 (Human)
Gene Symbol
MIER2
Additional MIER2 Products
Product Documents for MIER2 Antibody (OTI3G7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MIER2 Antibody (OTI3G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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