MIG2/Kindlin-2 Antibody (3A3)
Novus Biologicals | Catalog # NBP3-13846
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Hamster
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3A3
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Product Specifications
Immunogen
Recombinant protein containing MIG2/Kindlin-2 (residues 218-486) (Uniprot: Q96AC1)
Localization
Cytoplasm
Specificity
Kindlin-2 (also known as Mig-2, KIND2) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14275)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MIG2/Kindlin-2 Antibody (3A3)
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]](https://resources.rndsystems.com/images/products/MIG2-Kindlin-2-Antibody-3A3-Immunohistochemistry-Paraffin-NBP3-13846-img0001.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846] - Formalin-fixed, paraffin-embedded human liver stained with MIG2/Kindlin-2 antibody (3A3).![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]](https://resources.rndsystems.com/images/products/MIG2-Kindlin-2-Antibody-3A3-Immunohistochemistry-Paraffin-NBP3-13846-img0002.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846]
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (3A3) [NBP3-13846] - Formalin-fixed, paraffin-embedded human liver stained with MIG2/Kindlin-2 antibody (3A3).Applications for MIG2/Kindlin-2 Antibody (3A3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MIG2
Long Name
Mitogen Inducible Gene 2
Alternate Names
FERMT2, KIND2, Kindlin-2, PLEKHC1
Gene Symbol
FERMT2
UniProt
Additional MIG2 Products
Product Documents for MIG2/Kindlin-2 Antibody (3A3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MIG2/Kindlin-2 Antibody (3A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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