MIG2/Kindlin-2 Antibody (OTI9E4)

Novus Biologicals | Catalog # NBP1-47745

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E4
View all MIG2 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

77.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MIG2/Kindlin-2 Antibody (OTI9E4)

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

MIG2-Kindlin-2-Antibody-OTI9E4-Western-Blot-NBP1-47745-img0023.jpg
Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MIG2/Kindlin-2 Antibody (OTI9E4)

MIG2/Kindlin-2 Antibody (OTI9E4)[NBP1-47745]

MIG2/Kindlin-2 Antibody (OTI9E4)[NBP1-47745]Simple Western� analysis of endogenous protein HSP90AB1 from HELA lysates (0.2 mg/mL) using HSP90AB1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for MIG2/Kindlin-2 Antibody (OTI9E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIG2

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Long Name

Mitogen Inducible Gene 2

Alternate Names

FERMT2, KIND2, Kindlin-2, PLEKHC1

Entrez Gene IDs

10979 (Human)

Gene Symbol

FERMT2

Additional MIG2 Products

Product Documents for MIG2/Kindlin-2 Antibody (OTI9E4)

Certificate of Analysis

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Product Specific Notices for MIG2/Kindlin-2 Antibody (OTI9E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Integrins and Associated Molecules

Citations for MIG2/Kindlin-2 Antibody (OTI9E4)

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Protocols

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