Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99010

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Mineralocorticoid R/NR3C2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Mineralocorticoid R/NR3C2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010]

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010]

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010]

Immunoprecipitation: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010]

Immunoprecipitation: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-NR3C2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NR3C2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 107 kDaObserved band size: 107 kDa
Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] -

Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Staining of NR3C2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-99010 antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] -

Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] -

Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Simple Western lane view shows lysates of A172. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 107 kDa (as indicated) using 20 ug/mL of Mineralocorticoid R/NR3C2.

Applications for Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Application Notes
See Simple Western Antibody Database for Simple Western validation

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mineralocorticoid R/NR3C2

Steroid receptors are ligand dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain Mineralocorticoid Receptor.

Long Name

Mineralocorticoid Receptor

Alternate Names

MineralocorticoidR, MLR, NR3C2

Gene Symbol

NR3C2

Additional Mineralocorticoid R/NR3C2 Products

Product Documents for Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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