MITF Antibody (C5) - BSA Free

Novus Biologicals | Catalog # NB110-10872

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C5

Format

BSA Free
View all MITF Primary Antibodies »

Product Specifications

Immunogen

N-terminal fragment of human microphthalmia protein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Microphthalmia (C5) this antibody recognizes the 52/56 kD band and not the longer 60-70 kD proteins. Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52-56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for MITF Antibody (C5) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1 ug/ml

Immunoprecipitation

2 ug/mg protein lysate

Western Blot

1:500
Application Notes
IHC-P: Antigen retrieval is not essential but may optimise staining. WB: Detects a band of approximately 52-56 kDa. The antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Entrez Gene IDs

4286 (Human); 17342 (Mouse); 25094 (Rat)

Gene Symbol

MITF

UniProt

O75030 (Human)

Additional MITF Products

Product Documents for MITF Antibody (C5) - BSA Free

Certificate of Analysis

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Product Specific Notices for MITF Antibody (C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MITF Antibody (C5) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MITF Antibody (C5) - BSA Free

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  • Q: Has this antibody been used in ChIP application?

    A: We have not yet validated our MiTF antibody with catalogue number NB110-10872 for ChIP in house. We have however tested it for Western blotting, immunoprecipitation, and IHC with paraffin-embedded tissue samples.

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