Mitofusin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-51841

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mitofusin 1 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an N-terminal region of human Mitofusin-1 (within residues 5-100). [Swiss-Prot Q8IWA4]

Localization

Mitochondrion outer membrane; Multi-pass membrane protein.

Marker

Mitochondrial Fusion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mitofusin 1 Antibody - BSA Free

Western Blot: Mitofusin 1 AntibodyBSA Free [NBP1-51841]

Western Blot: Mitofusin 1 AntibodyBSA Free [NBP1-51841]

Mitofusin-1-Antibody-Western-Blot-NBP1-51841-img0010.jpg
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.
Immunohistochemistry: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunohistochemistry: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for Mitofusin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

reported in scientific literature
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-51841 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitofusin 1

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Alternate Names

hfzo1, hfzo2, MFN1

Entrez Gene IDs

55669 (Human)

Gene Symbol

MFN1

UniProt

Q8IWA4

Additional Mitofusin 1 Products

Product Documents for Mitofusin 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Mitofusin 1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Mitofusin 1 Antibody - BSA Free

Customer Reviews for Mitofusin 1 Antibody - BSA Free (1)

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  • Name: Vasundhra Bahl
    Application: Immunocytochemistry
    Sample Tested: Paraformeldehyde Fixed Cells
    Species: Mouse
    Verified Customer | Posted 03/28/2014
    Mfn-1 expressed in mouse neural stem cells
    Mitofusin 1 Antibody - BSA Free NBP1-51841

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Protocols

View specific protocols for Mitofusin 1 Antibody - BSA Free (NBP1-51841):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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