MKK3/MEK3 Antibody (2E12D11) - BSA Free

Name: MKK3/MEK3 Antibody (2E12D11) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52508
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52508

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 2E12D11

Format

BSA Free

View all MKK3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MKK3/MEK3 (AA: 1-138) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse MKK3/MEK3 Antibody (2E12D11) - BSA Free (NBP2-52508) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MKK3/MEK3 Antibody (2E12D11) - BSA Free

Western Blot: MKK3/MEK3 Antibody (2E12D11)BSA Free [NBP2-52508]

Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)

Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.

Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).

Western Blot: MKK3/MEK3 Antibody (2E12D11)BSA Free [NBP2-52508]

Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against HEK293 (1) and MAP2K3 (AA: 1-138)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of GC-7901 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunofluorescence: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of HepG2 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

ELISA: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

ELISA: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

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Applications for MKK3/MEK3 Antibody (2E12D11) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry

1:200-1:1000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000

Application Notes

This antibody is Cytof ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK3

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Gene Symbol

MAP2K3

Additional MKK3 Products

Product Documents for MKK3/MEK3 Antibody (2E12D11) - BSA Free

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Product Specific Notices for MKK3/MEK3 Antibody (2E12D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.