MKK3/MEK3 [p Ser189] Antibody - BSA Free

Name: MKK3/MEK3 [p Ser189] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-82048
Price: 229 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-82048

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human MKK3/MEK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).

Modification

p Ser189

Specificity

Detects endogenous levels of MKK3/MEK3 only when phosphorylated at serine189.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK3/MEK3 [p Ser189] Antibody - BSA Free

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] -

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048

Immunocytochemistry/Immunofluorescence: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] -

Immunocytochemistry/Immunofluorescence: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Staining of methanol-fixed Hela cells using NB100-82048

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048]

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) antibody

Immunohistochemistry-Paraffin: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] -

Immunohistochemistry-Paraffin: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82048(left) or the same antibody preincubated with blocking peptide (right).

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Applications for MKK3/MEK3 [p Ser189] Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100~1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK3

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Entrez Gene IDs

5606 (Human); 303200 (Rat)

Gene Symbol

MAP2K3

UniProt

P46734

Additional MKK3 Products

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Product Specific Notices for MKK3/MEK3 [p Ser189] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.