MKK6/MEK6 Antibody - BSA Free

Name: MKK6/MEK6 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-87791
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-87791

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK6/MEK6 Antibody - BSA Free

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791]

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for the same tissues.

Western Blot: MKK6/MEK6 Antibody [NBP1-87791]

Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunocytochemistry/ Immunofluorescence: MKK6/MEK6 Antibody [NBP1-87791]

Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human cell line A549 shows localization to nucleus & cytosol.

Western Blot: MKK6/MEK6 Antibody [NBP1-87791]

Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-341

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791]

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human colon shows high expression.

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791]

Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human testis shows low expression as expected.

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Applications for MKK6/MEK6 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK6

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4).

Long Name

Mitogen-activated Protein Kinase Kinase 6

Alternate Names

MAP2K6, MEK6, PRKMK6, SAPKK3

Gene Symbol

MAP2K6

Additional MKK6 Products

Product Documents for MKK6/MEK6 Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for MKK6/MEK6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.