Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MKRN2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 366 and the C-terminus (residue 416) of human makorin, ring finger protein, 2 using the numbering given in entry NP_054879.3 (GeneID 23609).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKRN2 Antibody - BSA Free

Western Blot: MKRN2 Antibody [NB100-55250]

Western Blot: MKRN2 Antibody [NB100-55250]

Western Blot: MKRN2 Antibody [NB100-55250] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-55250 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B), and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250]

Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250]

Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-MKRN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for MKRN2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MKRN2

The basic function of MKRN2 is mediating ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Makorin RING zinc finger-2 (MKRN2), overlaps and is antisense to the gene RAF1 in mammals. Northern blot analyses show that human MKRN2 and RAF1 are co-expressed in tissues and cell lines, raising the possibility of mRNA duplex formation. The encoded makorin-2 protein is likely a ribonucleoprotein of unknown function, but its conservation suggests an important cellular role.

Alternate Names

EC 6.3.2.-, HSPC070, makorin ring finger protein 2, probable E3 ubiquitin-protein ligase makorin-2, RNF62RING finger protein 62

Entrez Gene IDs

23609 (Human); 67027 (Mouse)

Gene Symbol

MKRN2

UniProt

Q9H000 (Human)

Additional MKRN2 Products

Product Documents for MKRN2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MKRN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MKRN2 Antibody - BSA Free

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Protocols

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