MMAB Antibody - BSA Free

Immunohistochemistry-Paraffin: MMAB Antibody [NBP1-86602]

Immunohistochemistry-Paraffin: MMAB Antibody [NBP1-86602] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Western Blot: MMAB Antibody - BSA Free [NBP1-86602]

Analysis in human cell line MCF-7 and human cell line U-2 OS.

Immunohistochemistry-Paraffin: MMAB Antibody - BSA Free [NBP1-86602]

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Western Blot: MMAB Antibody - BSA Free [NBP1-86602] -

Knockdown of MMAB increases LDLR expression and activity and decreases cholesterol biosynthesis in vitro.a Representative images of DiI-LDL uptake in Huh7 cells transfected with a non-silencing siRNA (NS) or siRNA MMAB (siMMAB) and incubated with 30 μg/ml DiI-LDL for 8 h at 37 C. Red, DiI-LDL; blue, nuclei stained with TO-PRO-3 iodide (TOPRO). Scale bar, 10 μm. Flow cytometry analysis of DiI-LDL specific binding and uptake in Huh7 cells transfected with NS siRNA or siMMAB and incubated with 30 μg/ml DiI-LDL for 8 h at 37 C (uptake) or 90 min at 4 C (binding) is shown below. Data are mean +/- s.e.m. of three independent experiments. Statistical comparisons by two-sided unpaired Student’s t test. b, c qRT-PCR (b) and Western analysis (c) of SREBP2-responsive genes in Huh7 cells transfected with an NS control siRNA or siMMAB. HSP90 was used as a loading control. Quantification compared to HSP90 is shown in panel (c). Data are mean +/- s.e.m. of three (c) or four (b) independent experiments in duplicate. Statistical comparisons by two-sided unpaired Student’s t test. d Sterol content of Huh7 cells transfected with NS siRNA or siMMAB. Lipids were extracted and analyzed by GC-MS. “Total sterols" were calculated as the sum of all sterols that could be detected. Data are mean +/- s.e.m. of three independent experiments. Statistical comparisons by two-sided unpaired Student’s t test. e Effect of MMAB knockdown on [1,2-14C]-acetate incorporation into cholesterol as determined by HPLC. Huh7 cells were treated as in (d). Data are representative of three independent experiments. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34750386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.