MMP-14/MT1-MMP Antibody (3-F7)

Novus Biologicals | Catalog # NBP2-67415

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3-F7 expressed in HEK293
View all MMP-14/MT1-MMP Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MMP-14/MT1-MMP aa 149-200 / 582. (SwissProt: P50281 Human; SwissProt: P53690 Mouse; SwissProt: Q10739 Rat)

Localization

Membrane, Melanosome, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-14/MT1-MMP Antibody (3-F7)

Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Human endometrial adenocarcinoma paraffin-embedded tissue section. Tissue section was fixed, deparaffinized and EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody. IHC-P image submitted by a verified customer review.
Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Applications for MMP-14/MT1-MMP Antibody (3-F7)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-67415 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (3-F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MMP-14/MT1-MMP Antibody (3-F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Citations for MMP-14/MT1-MMP Antibody (3-F7)

Customer Reviews for MMP-14/MT1-MMP Antibody (3-F7) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • MMP-14/MT1-MMP Antibody (3-F7)
    Name: Shinford Sun
    Application: Western Blot
    Sample Tested: Endothelial
    Species: Human
    Verified Customer | Posted 09/16/2024
    We examined MMP family members across various types of stromal cells, and the bands were mostly clear in the endothelial cells.
    MMP-14/MT1-MMP Antibody (3-F7) NBP2-67415
  • MMP-14/MT1-MMP Antibody (3-F7)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Endometrial adenocarcinoma
    Species: Human
    Verified Customer | Posted 10/17/2021
    Staining of endometrial adenocarcinoma tissue
    Fixed paraffin-embedded sections. Tissue sections were deparaffinized. EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody.
    MMP-14/MT1-MMP Antibody (3-F7) NBP2-67415

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