MMP-9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85575

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%). Human reactivity reported in scientific literature (PMID: 23897811).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-9 Antibody - BSA Free

MMP-9 Antibody - BSA Free Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
MMP-9 Antibody - BSA Free Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
MMP-9 Antibody - BSA Free Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
MMP-9 Antibody - BSA Free Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Staining of human kidney shows no positivity in cells in tubules as expected.
MMP-9 Antibody - BSA Free Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using HPA001238 antibody. Corresponding MMP9 RNA-seq data are presented for the same tissues.
MMP-9 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP1-85575]

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP1-85575]

Staining of human cell line U-2 OS shows localization to cytosol.

Applications for MMP-9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
WB reported in scientific literature (PMID: 23897811). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MMP-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMP-9 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A:

    We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

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