MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

Novus Biologicals | Catalog # NBP3-09101

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 15-2

Format

Low Endotoxin
View all MMR/CD206 Primary Antibodies »

Product Specifications

Immunogen

Purified human mannose receptor

Specificity

The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images for MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101]

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101]

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.
Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101]

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101]

Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Surface staining pattern of human human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.

Applications for MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Functional application: Blocking

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMR/CD206

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Gene Symbol

MRC1

Additional MMR/CD206 Products

Product Documents for MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

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Product Specific Notices for MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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