MMR/CD206/Mannose Receptor Antibody (BLR109H)

Novus Biologicals | Catalog # NBP3-14698

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR109H
View all MMR/CD206 Primary Antibodies »

Product Specifications

Immunogen

between 1406 and 1456 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for MMR/CD206/Mannose Receptor Antibody (BLR109H)

Immunoprecipitation: MMR/CD206/Mannose Receptor Antibody (BLR109H) [NBP3-14698]

Immunoprecipitation: MMR/CD206/Mannose Receptor Antibody (BLR109H) [NBP3-14698]

Immunoprecipitation: MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Whole celllysate (1.0 mg per IP reaction; 20% of IP loaded) fromMUTZ-3 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-CD206/Mannose Receptorrecombinant monoclonal antibody used for IP at 6 ul per reaction. CD206/MannoseReceptor was also immunoprecipitated by rabbit antiCD206/Mannose Receptor antibody. Forblotting immunoprecipitated CD206/Mannose Receptor, was used at 1:1000. Chemiluminescence withan exposure time of 10 seconds.
MMR/CD206/Mannose Receptor Antibody (BLR109H) MMR/CD206/Mannose Receptor Antibody (BLR109H)

MMR/CD206/Mannose Receptor Antibody (BLR109H)- Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit antiCD206/Mannose Receptor recombinant monoclonal antibody [BLR109H]used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody

MMR/CD206/Mannose Receptor Antibody (BLR109H)- Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit antiCD206/Mannose Receptor recombinant monoclonal antibody [BLR109H]used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody
MMR/CD206/Mannose Receptor Antibody (BLR109H) MMR/CD206/Mannose Receptor Antibody (BLR109H)

MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
MMR/CD206/Mannose Receptor Antibody (BLR109H) MMR/CD206/Mannose Receptor Antibody (BLR109H)

MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

MMR/CD206/Mannose Receptor Antibody [NBP3-14698] - Rabbit antiCD206/Mannose Receptor recombinant monoclonalantibody [BLR109H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Applications for MMR/CD206/Mannose Receptor Antibody (BLR109H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

6 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: MMR/CD206

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Gene Symbol

MRC1

Additional MMR/CD206 Products

Product Documents for MMR/CD206/Mannose Receptor Antibody (BLR109H)

Certificate of Analysis

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Product Specific Notices for MMR/CD206/Mannose Receptor Antibody (BLR109H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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