Moesin Antibody (MSN/491) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-34685
![Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Western-Blot-NBP2-34685-img0011.jpg "Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]")
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MSN/491
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038)
Localization
Cytoplasmic & Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32875).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Moesin Antibody (MSN/491) - Azide and BSA Free
Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]
Western Blot: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).![Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34685-img0010.jpg "Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34685-img0006.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin Rat colon stained with Moesin MAb (MSN/491)![Flow Cytometry: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34685-img0007.jpg "Flow Cytometry: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Flow Cytometry: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Flow Cytometry: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Flow Cytometric Analysis of K562 cells using Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Western-Blot-NBP2-34685-img0004.jpg "Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]")
Western Blot: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]
Western Blot: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Western blot of HT29 Cell Lysate using Moesin MAb (MSN/491).![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34685-img0002.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin human placenta stained with moesin MAb (MSN/491)![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34685-img0003.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin human Melanoma stained with moesin MAb (MSN/491)![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34685-img0005.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]")
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685]
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin Rat lung stained with Moesin MAb (MSN/491)![Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Simple-Western-NBP2-34685-img0008.jpg "Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]")
Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]
Simple Western: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Simple Western lane view shows a specific band for Moesin in 0.1 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]](https://resources.rndsystems.com/images/products/Moesin-Antibody-MSN-491-Azide-and-BSA-Free-Simple-Western-NBP2-34685-img0009.jpg "Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]")
Simple Western: Moesin Antibody (MSN/491)Azide and BSA Free [NBP2-34685]
Simple Western: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Electropherogram image of the corresponding Simple western lane view. Moesin antibody was used at 1 ug/ml dilution on HUVEC lysate(s) respectively.Applications for Moesin Antibody (MSN/491) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Moesin
Alternate Names
Membrane-organizing extension spike protein, moesin
Gene Symbol
MSN
Additional Moesin Products
Product Documents for Moesin Antibody (MSN/491) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Moesin Antibody (MSN/491) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Moesin Antibody (MSN/491) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Moesin Antibody (MSN/491) - Azide and BSA Free and earn rewards!
Have you used Moesin Antibody (MSN/491) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Moesin Antibody (MSN/491) - Azide and BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
A: The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.
Loading...