Moesin Antibody (SPM562) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-44578

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM562

Format

IHC-Prediluted
View all Moesin Primary Antibodies »

Product Specifications

Immunogen

Moesin Purified from uterus (Uniprot: P26038)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Moesin Antibody (SPM562) - IHC-Prediluted

Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578]

Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578]

Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578] - Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Antibody (SPM562)
Moesin Antibody (SPM562) - IHC-Prediluted Western Blot: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578] -

Western Blot: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578] -

Western Blot Analysis of PC3 cell lysate. Moesin Antibody (SPM562) - IHC-Prediluted.
Moesin Antibody (SPM562) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578] -

Immunocytochemistry/ Immunofluorescence: Moesin Antibody (SPM562) - IHC-Prediluted [NBP2-44578] -

Immunofluorescence Analysis of PFA fixed HeLa cells labeling Moesinwith Moesin Antibody (SPM562) - IHC-Prediluted followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Applications for Moesin Antibody (SPM562) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Moesin

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Alternate Names

Membrane-organizing extension spike protein, moesin

Gene Symbol

MSN

Additional Moesin Products

Product Documents for Moesin Antibody (SPM562) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Moesin Antibody (SPM562) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Moesin Antibody (SPM562) - IHC-Prediluted

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  • Q: I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.

    A: The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

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