Moesin Antibody (SPM562) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34798

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM562

Format

Azide and BSA Free
View all Moesin Primary Antibodies »

Product Specifications

Immunogen

Moesin Purified from uterus (Uniprot: P26038)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32876).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Moesin Antibody (SPM562) - Azide and BSA Free

Western Blot: Moesin Antibody (SPM562)Azide and BSA Free [NBP2-34798]

Western Blot: Moesin Antibody (SPM562)Azide and BSA Free [NBP2-34798]

Western Blot: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798] - Western Blot Analysis of PC3 cell lysate. Moesin Mouse Monoclonal Antibody (SPM562).
Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798]

Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798]

Immunohistochemistry-Paraffin: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798] - Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Antibody (SPM562)
Moesin Antibody (SPM562) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798] -

Immunocytochemistry/ Immunofluorescence: Moesin Antibody (SPM562) - Azide and BSA Free [NBP2-34798] -

Immunofluorescence Analysis of PFA fixed HeLa cells labeling Moesinwith Moesin Antibody (SPM562) - Azide and BSA Free followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Applications for Moesin Antibody (SPM562) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Moesin

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Alternate Names

Membrane-organizing extension spike protein, moesin

Gene Symbol

MSN

Additional Moesin Products

Product Documents for Moesin Antibody (SPM562) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Moesin Antibody (SPM562) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Moesin Antibody (SPM562) - Azide and BSA Free

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  • Q: I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.

    A: The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

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