Mohawk homeobox Antibody (OTI6B12)

Novus Biologicals | Catalog # NBP2-45863

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6B12
View all Mohawk homeobox Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MKX(NP_775847) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mohawk homeobox Antibody (OTI6B12)

Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mohawk homeobox.
Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Immunohistochemistry: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Mohawk-homeobox-Antibody-OTI6B12-Immunohistochemistry-NBP2-45863-img0006.jpg
Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863]

Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for Mohawk homeobox Antibody (OTI6B12)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 4 using NBP2-45863 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mohawk homeobox

MKX is a member of an Iroquois (IRX) family-related class of 'three-amino acid loop extension' (TALE)atypical homeobox proteins characterized by 3 additional amino acids in the loop region between helix I and helix IIof the homeodomain (Anderson et al., 2006 (PubMed 16408284)).(supplied by OMIM)

Alternate Names

C10orf48, homeobox protein Mohawk, IFRX, Iroquois family related homeodomain protein, iroquois homeobox protein-like 1, IRXL1chromosome 10 open reading frame 48, MGC39616, mohawk homeobox

Gene Symbol

MKX

Additional Mohawk homeobox Products

Product Documents for Mohawk homeobox Antibody (OTI6B12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mohawk homeobox Antibody (OTI6B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mohawk homeobox Antibody (OTI6B12)

Customer Reviews for Mohawk homeobox Antibody (OTI6B12) (1)

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Customer Images

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  • Mohawk homeobox Antibody (6B12)
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell lysate (human dermal fibroblasts - HDFa)
    Species: Human
    Verified Customer | Posted 01/22/2018
    The antibody worked well in the sandwich ELISA. Regarding the OD, it has been read at 450nm (TMB monitored at 620nm and then stop solution to read at 450nm)
    Capture antibody: anti-MKX antibody NBP2-45863 (2ug/mL) - overnight incubation at 4ºC Protein: Recombinant human Mohawk homeobox protein from another supplier at different concentrations (62,5-2000ng/ml) Detection: Rabbit pAb to Mohawk homeobox from another supplier - 1/100 1h at room temperature Secundary: Goat pAb to Rb IgG (HRP) from another supplier - 1/5000 1h at room temperature Sample type: Cell lysate (human dermal fibroblasts - HDFa)
    Mohawk homeobox Antibody (OTI6B12) NBP2-45863

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Protocols

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